Flow Cytometry Protocols

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Mark J. Jaroszeski, Richard Heller
Humana Press, 1998 - Science - 274 pages
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In Flow Cytometry Protocols, leading researchers and experimenters update the complex and technically sophisticated field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and a method that combines FISH with flow cytometry. Advanced methods for chromosome sorting and 5-6 color analysis are also detailed. Flow Cytometry Protocols contains methods that take advantage of the recently developed instrumentation and the vast array of novel probes that have led to advances in the field of flow cytometry, as well as to significantly improved standard methods. This eminently practical new book will play a key role in bringing researchers up-to-date in this unique and productive technology.

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