Culture of Animal Cells: A Manual of Basic Technique
Cultured!cell - Biohazards - Sterilization - Cell!lines - Cloning - Specific!cell!types - Cell!separation - Transformed!phenotype - Cytotoxicity - Culture!of!specific!cell!types - Culture!of!tumor!tissue - Three!dimensional!culture!systems.
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Biology of the Cultured Cell
Design and Layout of the Laboratory
Equipping the Laboratory
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acid agar ampules antibody assay autoclave autoradiography Bellco bottle buffer cell counting cell culture cell density cell lines cell suspension cell types centrifuge Chapter chromosome cloning collagen collagenase colonies concentration containing continuous cell lines coverslip culture flasks deionized differentiation dilute disaggregation EDTA embryo enzyme epithelial cells epithelium explant feeder layer fetal fibroblasts filter filtration fluorescence freezer GIBCO glass glassware growth factors growth medium HBSS hemocytometer hormones human incubator laboratory laminar flow laminar flow cabinet liquid melanocytes methanol microscope monolayer mouse normal organ culture Pasteur pipette PBSA petri dishes phase pipette plastic plating efficiency preparation primary culture proliferation protein Protocol reagents remove resuspend rinse room temperature sample sedimentation seed selection serum serum-free Sigma slide solution stain sterile stirrer storage subculture substrate supernatant surface technique tion tissue culture transfer transformed trypsin tube tumor tumor cells virus vitro vivo wash