Culture of Animal Cells: A Manual of Basic TechniqueCultured!cell - Biohazards - Sterilization - Cell!lines - Cloning - Specific!cell!types - Cell!separation - Transformed!phenotype - Cytotoxicity - Culture!of!specific!cell!types - Culture!of!tumor!tissue - Three!dimensional!culture!systems. |
Common terms and phrases
acid agar aliquots ampules antibody assay autoclave autoradiography Becton Dickinson Bellco bottle buffer cell counting cell culture cell density cell lines cell suspension cell types centrifuge chamber Chapter chromosome cloning cm² collagen collagenase colonies concentration containing continuous cell lines coverslip differentiation dilution disaggregation drug EDTA embryo enzyme epithelial cells epithelium explant feeder layer fetal fibroblasts filter flow fluorescence freezer GIBCO glass gradient growth factors growth medium HBSS hemocytometer human incubator laboratory liquid Materials Protocol melanocytes methanol method microscope microtitration monolayer mouse mycoplasma normal number of cells organ culture P.O. Box Pasteur pipette PBSA petri dishes phase pipette plastic plating efficiency prepared primary culture proliferation protein Remove resuspend rinse sample sedimentation seed selective serum serum-free Sigma slide solution stain sterile subculture substrate supernatant surface technique tion tissue culture transfer transformation trypsin tube tumor tumor cells virus vitro wash µg/ml