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0-glucans absorbance acetate acid assay added alginate alkali amino ammonium analysis angle approx aqueous automated Biochem Biochemistry Biol Biophys buffer butanol c.d. bands c.d. behaviour carbon cell cellulose chain conformation characterisation Chem chromatography chromophore circular dichroism coil concentration containing D-glucose debubbler degradation detergent determined electron enzymes ethanol extraction films Flow rates galactomannans glucan glucose glycogen glycoproteins glycosides grids heating hydrolysis intrinsic viscosity Kleinschmidt length linkage lipid measured membrane method Mg/ml ml/min molar ellipticity molecular weight molecules monosaccharides Morris NADP NaOH neuraminic acid Northcote nucleotide oligosaccharides optical activity parameters pectin peptide periodate oxidation phosphate polygalacturonic polymer polyprenol derivative polysaccharides PPSs protein radioactive range reaction reagent Rees residues rotation ruthenium red sample Section sensitivity shear rate sodium solution solvent specific spectra spectrum staining starch step structure sucrose sugars Techniques in Carbohydrate temperature tion tissue transitions tubes uronic acids viscometer wavelength