Manual of Industrial Microbiology and Biotechnology, Volume 52Arnold L. Demain, Nadine A. Solomon This second edition of the best-selling Manual of Industrial Microbiology and Biotechnology (MIMB) brings together in one place the platform biological and engineering methodologies required to develop a successful industrial process through culture isolation and development. It describes the means by which traditional industrial microbiology and biotechnology, as well as modern technology, can be carried out in academic or industrial laboratories, pilot plant, or factory. The editors have enlisted a broad range of experts, including microbial ecologists, physiologists, geneticists, biochemists, molecular biologists, and biochemical engineers, who offer practical experience not found in texts and journals. This comprehensive perspective makes MIMB a valuable "how to" resource, the structure of which resembles the sequence of operation involved in the development of a commercial biological process and product. |
Contents
Isolation of Cultures J C HUNTERCEVERA M E FONDA and Angela Belt | 3 |
Screening for New Products from Microorganisms RICHARD J White William M Maiese | 24 |
Inoculum Development G R HUNT AND R W STIEBER | 32 |
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acid actinomycetes activity agar agitation amino anaerobic antibiotic Appl assay autoclave auxotrophs bacteria batch Biochem Bioeng bioreactor Biotechnol broth buffer carbon cell culture centrifuge cephamycin cerevisiae Chem chemical chemostat chitin chromatography cloning coli colonies compounds concentration containing cost dilution Distilled water enzyme equation equipment Escherichia coli estimate ethanol fermentation fermentor filter flasks fungi gene genetic glucose growth heat immobilized incubation industrial inoculated inoculum isolation laboratory liquid liter materials medium membrane ment method microbial cells Microbiol microbiology microorganisms mixing mutants nitrogen nutrient nystatin obtained operation organisms oxygen penicillin phosphate pilot plant plasmid plates preparation procedures production protein protoplast fusion protoplasts reactions recombinant redox regeneration resin sample scale-up screen selection shaker sodium solid solution solvent specific spores sterile strain Streptomyces substrate suspension Table techniques temperature tion tissue transfer tube variables vessel volume µg/ml