Procedures in Nucleic Acid Research, Volume 2 |
Contents
Raman Spectroscopy of Polynucleotides and Nucleic Acids | 94 |
Dichroism | 176 |
H TODD MILES Laboratory of Molecular Biology National Institute of Arthritis | 205 |
Copyright | |
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absorbance absorption Acad acceptor acetate Acta activity amino acid aminoacyl-tRNA ammonium sulfate aqueous assay band BD-cellulose binding Biochem Biochemistry Biol Biophys Biopolymers buffer pH buoyant density cell cellulose centrifugation Chem chromatography cm¹ coefficient column column chromatography complex components concentration containing Cotton effects countercurrent distribution CsCl curve denatured density gradient described determined dialyzed dichroism digestion diluted EDTA eluted enzyme equilibrated equilibrium ethanol fluorescence fraction gm/cm³ hydrogens hydroxyapatite incubation interaction isostichs labeled linear macromolecules measured method MgCl2 mixture molarity molecular weight molecules NaCl Natl nucleic acids nucleotides obtained optical phage phosphate poly polymer polymerase polynucleotide precipitate preparation Proc procedure protein protons purified purine pyrimidine radioactivity Raman ratio reaction repressor ribonuclease salt sample sedimentation separation sodium sodium acetate solution solvent specific spectra spectrum structure Szybalski technique temperature Tris-HCl tRNA tube values vibrations volume wavelength yeast