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addition amino acid arginase assay arginine and lysine Arginine concentration arginine requirement argininosuccinase Basal Basal basal diet supplemented Biochem Biol Biophys buffer solution carbamyl phosphate centrifugation Chem chick kidney arginase chick liver chickens selected citrulline concentration was varied concentrations for curves creatine dialysis dietary arginine dietary excesses differential centrifugation effect of AIB effect of lysine effect of ornithine enzyme assay enzyme prepared fed the basal glutamic acid glycine heat treatment incubation intracellular localization isolated from kidneys isozymes kidney arginase activity kidney arginase prepared kinetic Km values lysine lysosomes malate dehydrogenase mammary gland marker enzymes metabolism mitochondrial fraction mitochondrial matrix MnCl2 NESHEIM non-sonicated Nutr observed obtained optimum pH oxidase partially purified enzyme procedure proline protein rat kidney rat liver arginase reaction Recovery sonicated specific activity strain chicks fed studies substrate concentration sucrose supernatant Table tissue Total units Tris-HCl buffer urea cycle ureotelic animals uricotelic varied from 0.+5