Bioseparation and Bioprocessing: Processing, quality and characterization, economics, safety and hygieneG. Subramanian |
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activity amino acid analysis antibody application assays batch bead mill Biochem Biol Chem biological bioprocessing Biotechnol Bioeng Biotechnology buffer C-terminal carbohydrate carbohydrate chains carriers cell culture cell debris cell disruption cell lines centrifuge chemical chromatography coli column concentration containment cytokines deamidation degradation denaturation density disulfide bonds downstream processing Edman Edman degradation effect enzymatic enzymes equipment factors fermentation filters filtration fluidized bed fraction function glycans glycoprotein glycosylation homogenizer HPLC human IFN-a immobilized inclusion bodies increase insulin Interferon ions MAbs macroporous MALDI mass medium membrane methods microcarriers molecular molecules oligosaccharides operations oxygen packed bed parameters peptide peptidoglycan polypeptide potency potential proteases protein purification reaction reactor recombinant recovery redox reduced refolding release residues sample scale scale-up sequence soluble solution solvent specific stability standard step sterilization structure surface techniques temperature therapeutics tion valve velocity yeast yield