Animal cell culture: a practical approach
Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetricindicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presentingtechniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.
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Introduction to basic principles
Towards serumfree chemically defined
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acid agar ampoules antibody antigens assay attachment Biol bioreactor buffer Cancer carbon dioxide cell culture cell density cell growth cell lines cell numbers cell suspension cell type centrifugation chamber cloning collagen concentration containing culture vessel Cytotechnology cytotoxicity diameter differentiation dilution distilled water drug elutriation embryos enzyme epithelial ethanol explants fibroblasts fibronectin Figure filter flask flow cytometry glass growth factors growth medium HBSS hormones human hybridization hybridomas increase incubation insulin laboratory lipids litre membrane method microcarrier monoclonal antibodies monolayer mycoplasma organ culture oxygen Pasteur pipette perfusion phase pipette plasma plastic plates preparation probe procedure production proliferation protein Protocol reagents remove rinse room temperature sample scale-up Section serum serum-free slides solution specific staining sterile stirring substrate suitable surface area suspension culture technique tion transfer transferrin trypsin tube tumour vitro Vitro Cell volume wash