Animal Cell Culture: A Practical ApproachR. Ian Freshney Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetricindicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presentingtechniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture. |
Contents
Towards serumfree chemically defined | 15 |
Strategies for designing serumfree cultures and appropriate | 21 |
Procedures for coating of culture surface with biomatrix | 41 |
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acid agar ampoules antibody antigens assay autoradiography Biol buffer Cancer Res carbon dioxide cell culture cell lines cell number cell population cell suspension cell type centrifugation chamber cloning clot concentration containing culture vessel cytotoxicity diameter diluted distilled water droplets drug EDTA elutriation embryos enzyme epithelium ethanol explants exposure Figure filter flow cytometry fluid fluorescence glass growth factors growth medium HBSS hormones Human Tumour hybridization hybridomas incubation labelled laboratory light measure membrane method microcarrier microscope monolayer mycoplasma number of cells organ culture oxygen Pasteur pipettes perfusion Petri dish pipette plasma plastic plates Preparation probe procedure proliferation propidium iodide protein Protocol quantitation Reagents remove rinse room temperature rotor sample serum serum-free slides solution specific spheroids staining sterile surface technique tissue transfer trypsin tube viability vitro vivo wash watchglass µg/ml