Animal Cell Culture: A Practical ApproachR. Ian Freshney Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture. |
Contents
Towards serumfree chemically defined | 15 |
Strategies for designing serumfree cultures and appropriate | 21 |
Practical suggestions for the preparation and handling | 38 |
Copyright | |
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acid agar antibody assay autoradiography Biol buffer Cancer Res carbon dioxide cell culture cell density cell lines cell number cell population cell suspension cell types centrifuge chamber cloning clot concentration containing culture vessel cytotoxicity diameter differentiation dissecting distilled water droplets drug elutriation embryos enzyme epithelium ethanol explants exposure filter flow cytometry flow rate fluid fluorescence forceps fraction glass growth factors growth medium HBSS hormones Human Tumour hybridization hybridomas incubation laser litre measure membrane method microcarrier microscope monolayer number of cells obtained organ culture oxygen Pasteur pipettes perfusion Petri dish pipette plasma plastic plates prepared procedure proliferation propidium iodide protein Protocol pump quantitation Reagents remove rinse room temperature rotor sample sensitivity serum serum-free slides solution specific spheroids staining sterile surface technique tissue transfer trypsin tubes viability vitro vivo wash watchglass wavelength µg/ml