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_ Fig 24 hours 95 per cent absolute alcohol acetone alum ammonium balsam block Blot bottle c.c. distilled water carbolxylol carbonate celloidin celloidin sections cells cent alcohol cent aqueous solution cent formaldehyde cent solution Clear in xylol color counterstain cover glass Cut frozen sections Dehydrate Differentiate dilute Dissolve distilled water drops edge embedding eosin fibers filter paper Fix tissues fixation formaldehyde fuchsin gentian violet glycerin granules gum dammar gum mastic hematoxylin hydroxide iodine isopropanol Kaiserling knife Krajian mastic methyl methylene blue microscope microtome mixture Mount in gum nuclei osmic acid paraffin paraffin oven paraffin sections picric acid Place potassium precipitate prepared pure xylol reagent remove reticulum running water safranine silver nitrate slide smears sodium sodium hyposulfite specimen Spirochaeta staining methods stock solution strop sulfate tap water thin tion usual manner violet Wash in distilled Wash in tap Wash in water xylol Zenker's fluid