RNA - Ligand Interactions, Part A: Structural Biology MethodsRNA-Ligand Interactions, Part A focuses on structural biology methods. Major topics covered include semisynthetic methodologies (RNA synthetic methods and derivatization of RNA); RNA structure determination (X-ray crystallography, NMR, EM); techniques for monitoring RNA conformation and dynamics (solution methods and electrophoretic and spectroscopic methods); and modeling tertiary structure: Part B, its companion Volume 318 of Methods in Enzymology, focuses on molecular biology methods. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the Series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. |
Contents
RNA Structure Determination | 147 |
Techniques for Monitoring RNA Conformation and Dynamics | 311 |
Modeling Tertiary Structure | 455 |
Common terms and phrases
acceptor acetate AcPhe-tRNA activity addition alignment analogs analysis antibiotic assay binding Biochem Biochemistry Biol buffer Cell Chem chemical chromatography circular permutation cleavage cleaved complex concentration conformational containing CP isomers crystal density dipolar couplings DNA ligase DNAzyme donor Edited electron eluted enzyme ethanol experiments fluorescence fluorophore folding function hammerhead ribozyme heavy atom helical helix incubated interactions intron ions isomers K. H. Nierhaus labeled ligand ligase ligation Methods Enzymol MgCl2 mmol modified molecular mutant Natl NTPs Nucleic Acids Nucleic Acids Res nucleotides O. C. Uhlenbeck observed oligonucleotide phage phosphate phosphoramidite phosphorothioate polymerase position preparation probe procedure protein protons purified pyridine quench reagent residual dipolar couplings resonances ribose ribosome RNA molecules RNA structure RNase H room temperature sample secondary structure sequence solution specific spectra studies substrate synthesis T. R. Cech template tertiary tion transcription tRNA urea VOLUME X-ray yield