Eukaryotic MRNA Processing
New York, 1997 - ARN messager - 378 pages
This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
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The chemistry of selfsplicing or selfcleaving RNA
Kinetics of RNA catalysis
Are group II and nuclear mRNA introns evolutionarily related?
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activity addition alternative splicing analysis appears assembly associated base base-pairing binding biochemical Biol catalytic Cell Chapter characterization cleavage complex components conserved contains core cytoplasm demonstrated determined domain downstream Drosophila editing efficiency elements EMBO encoding enhancer essential evidence example exon expression extracts formation function Genes Dev genetic gRNAs hnRNP human identified indicate interactions intron involved isolated localization mammalian mechanism molecules motif mRNA mutations Natl Acad Nature Nucleic Acids Res nucleotides observed occurs pairing particles poly(A polyadenylation polymerase pre-mRNA splicing present Proc processing reaction recently recognition region regulation revealed reviewed ribozyme RNA polymerase II role Science selection sequence showed shown similar snRNA snRNP proteins specific splice site splice sites spliceosome splicing factor step structure studies substrate subunit suggested tetramers trans-splicing transcripts U1 snRNP U6 snRNA upstream vitro vivo yeast