Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-29
... tube in a boiling - water bath for exactly 40 seconds . 4. Centrifuge the bacterial lysate at 12,000g for 10 minutes at room temperature in a microfuge . 5. Remove the pellet of bacterial debris from the microfuge tube with a sterile ...
... tube in a boiling - water bath for exactly 40 seconds . 4. Centrifuge the bacterial lysate at 12,000g for 10 minutes at room temperature in a microfuge . 5. Remove the pellet of bacterial debris from the microfuge tube with a sterile ...
Page 1-40
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 M NaCl containing 13 % ( w / v ) ...
... tube , and add 3 ml of an ice - cold solution of 5 м LiCl . Mix well , and then centrifuge the solution at 10,000 ... microfuge tube and store at room temperature for 30 minutes . 5. Add 500 μl of 1.6 M NaCl containing 13 % ( w / v ) ...
Page 4-29
... microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the supernatant to a fresh microfuge tube , and add 200 μl of a solution of 20 % polyethylene glycol ( PEG 8000 ) in 2.5 ...
... microfuge tube , and centrifuge at 12,000g for 5 minutes at 4 ° C in a microfuge . 3. Transfer about 1.2-1.3 ml of the supernatant to a fresh microfuge tube , and add 200 μl of a solution of 20 % polyethylene glycol ( PEG 8000 ) in 2.5 ...
Contents
Plasmid Vectors | xi |
DNA METHYLATION | xvii |
Gel Electrophoresis of | xix |
Copyright | |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage M13 bacteriophage particles bacteriophage T4 DNA BamHI buffer cDNA cells chloramphenicol cohesive termini coli concentration containing cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA Polymerase EcoRI EDTA efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA extracts foreign DNA gene gradients HindIII Hindill host hybridization Incubate infected inserted Kpnl lacZ libraries ligation ligation reaction linear lysis lysogenic method microfuge tube minutes at 4°C molecular cloning nin5 nitrocellulose nitrocellulose filters Nucleic Acids Oligonucleotides origin of replication packaging pellet phagemid plaques plasmid DNA plasmid vectors polycloning prepared probe protein protocol Purification Pvul Radiolabeled recA red gam replication restriction enzymes room temperature Sacl Sall segment of foreign single-stranded DNA Smal solution Southwestern Medical Center sterile stored strains strand supernatant T4 DNA ligase teriophage Texas Southwestern Medical Transfer transformation Tris Cl pH vector DNA Xbal µg/ml