Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 24
... culture of bacteria . Growing and harvesting a bacterial culture Most bacteria can be grown without too much difficulty in a liquid medium ( broth culture ) . The culture medium must provide a balanced mixture of the essential nutrients ...
... culture of bacteria . Growing and harvesting a bacterial culture Most bacteria can be grown without too much difficulty in a liquid medium ( broth culture ) . The culture medium must provide a balanced mixture of the essential nutrients ...
Page 37
... culture incubated for a further 12 hours . During this time the plasmid molecules continue to replicate , even ... culture ( bacteria + extracellular phage ) Phage suspension 888 Pellet of bacteria Figure 3.14 Preparation of a phage ...
... culture incubated for a further 12 hours . During this time the plasmid molecules continue to replicate , even ... culture ( bacteria + extracellular phage ) Phage suspension 888 Pellet of bacteria Figure 3.14 Preparation of a phage ...
Page 38
An Introduction Terence A. Brown. bacterial culture . When such a culture is centrifuged , the bacteria are pelleted , leaving the phage particles in suspension ( Fig . 3.14 ) . The phage particles are then collected from the suspension ...
An Introduction Terence A. Brown. bacterial culture . When such a culture is centrifuged , the bacteria are pelleted , leaving the phage particles in suspension ( Fig . 3.14 ) . The phage particles are then collected from the suspension ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein