Gene Cloning: An IntroductionThird edition. This concise and accessible introduction to gene cloning assumes almost no background knowledge. |
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Page 98
... polylinker is designed so that it does not totally disrupt the lacZ ' gene ; a reading frame is maintained throughout the polylinker , and a functional , though altered , B - galactosidase enzyme is still produced ( Fig . 6.7b ) . When ...
... polylinker is designed so that it does not totally disrupt the lacZ ' gene ; a reading frame is maintained throughout the polylinker , and a functional , though altered , B - galactosidase enzyme is still produced ( Fig . 6.7b ) . When ...
Page 99
... polylinker is reinserted , and the original M13mp7 reformed , then blue plaques will result . But what if the vector self - ligates , with neither new DNA nor the polylinker inserted ? Once again the design of the polylinker comes into ...
... polylinker is reinserted , and the original M13mp7 reformed , then blue plaques will result . But what if the vector self - ligates , with neither new DNA nor the polylinker inserted ? Once again the design of the polylinker comes into ...
Page 100
... polylinker . More complex M13 vectors The latest M13 vectors have more complex polylinkers inserted into the lacZ ' gene . An example is M13mp8 ( Fig . 6.10a ) , which is the counterpart of the plasmid pUC8 ( see p . 95 ) . As with the ...
... polylinker . More complex M13 vectors The latest M13 vectors have more complex polylinkers inserted into the lacZ ' gene . An example is M13mp8 ( Fig . 6.10a ) , which is the counterpart of the plasmid pUC8 ( see p . 95 ) . As with the ...
Contents
Preface | 1 |
plasmids and bacteriophages | 13 |
Purification of DNA from living cells | 25 |
Copyright | |
12 other sections not shown
Common terms and phrases
Adaptors agar amino acid ampicillin animal antibiotic antibody B-galactosidase bacterial cells bacteriophage bacterium BamHI base pairing biotechnology blunt ended carried cDNA cell extract centrifugation Chapter cleavage cleave cloned gene cloning vectors coded coli coli cells colonies contain copy number cosmid culture density digestion DNA fragments DNA ligase DNA sequencing double-stranded EcoRI electrophoresis enzyme expression vector Figure gene cloning gene cloning experiment host cell hybridization probing insulin intron labelled lacZ leu2 ligation lysed medium method mitochondrial Molecular Biology mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide organisms packaging phage DNA phage particles plant cells plaques plasmid DNA polylinker polymerase polynucleotide preparation produced promoter pUC8 purified recognition sequences recombinant DNA recombinant DNA molecule recombinant protein replication restriction endonucleases restriction sites resulting segment specific sticky ends strand structure synthesis T-DNA techniques tetracycline Ti plasmid transformed translation product trpA types unique restriction virus vitro yeast zein