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acetate acetone alcoholic extract allowed to evaporate aluminum amorphous material amyl alcohol analysis appear as carbonate aqueous extract ash by Morin ash content ash determination bitter principle bitter taste calcium malate calcium oxalate Carduus benedictus cent Chim chloroform cnicin obtained Cnicus benedictus color crystalline material crystals separated crystals were obtained digitaline dissolved E. R. Miller ether and ether ether solution extracted with petroleum Fehling's solution filtered filtrate flask formula Frickhinger 1853 Geiseler glucoside gram of cnicin grams of drug grams of powder H. A. Langenhan Haider heated heptane hydrogen large amount Liquor Potassii Arsenitis magnesium malate melting point methyl alcohol Nativelle Nellie Wakeman old extract petroleum ether phosphate picric acid plant Potassae Arsenitis U. S. P. potassium carbonate potassium chloride potassium nitrate purified reported residue resinous Rosenthaler and Stadler salicin saponification value Schwandner Scribe small amount sodium Soltmann soluble stems substance tion total ash treated volatile oil yielded