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acetone acid methyl esters Acta adsorbent adsorption alumina amounts Anal analysis analyzed application aqueous benzene bile acid methyl bile acids Biochem Biol biological Chem chloroform cholanic cholestane cholesterol cholic Chromatog components concentration conjugates containing derivatives detection diethyl ether E. C. Horning eluate elution esters ethanol ethyl acetate extraction fraction gas chromatography gel G hexane hydrocarbons hydroxyl impregnated isomers lathosterol Lipid Res liquid phases lithocholic metabolites method methyl mixture mobile phase obtained oxidation paper chromatography partition peak petroleum ether plasma plates polar prepared procedure prostaglandins purification quantitative radioactivity reaction reagent recovery residue retention retinene retinoic acid retinol retinyl palmitate reversed-phase Rf values sample saturated separation silica gel silica gel layers silicic acid silyl ethers Sjovall solution solvent solvent systems Spray stationary phase steroids sterols substances technique temperature testosterone thin-layer chromatography tion tissue tocopherols trifluoroacetates trimethyl silyl ethers ultraviolet vitamin A compounds