Studies on the Effect of Calcium, Lithium and Iodine on the Growth of Rhizobia |
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107-1 in synthetic 205 in synthetic 318 in synthetic 50 ug/liter 500 ml side-arm added calcium ammonium nitrate amount of growth Azotobacter bacteroid calcium added calcium chloride calcium deficient medium calcium nitrate calcium requirement cells concentrations of calcium concentrations of lithium cultures in side-arm deficient in calcium effect of calcium effect of iodine effect of lithium final pH fixing nitrogen following modifications growth of rhizobia growth rate high concentrations incubation hr iodine as potassium lag phase legumes lithium as lithium lithium chloride lithium salts logarithmic phase mannitol maximum growth McCalla medium without calcium ml side-arm flask mu band nitrogen fixation nodules Omitting calcium pantothenate Optical density pantothenate and calcium potassium iodide potassium nitrate ppm of calcium ppm of iodide reported rhizobia strains Rhizobium meliloti 107-1 Rhizobium trifolii 205 Schwinghamer's synthetic medium Shaken cultures side-arm flask shaken species of rhizobia spheroplasts strains of rhizobia TABLE total amount total growth ug of biotin