Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques
In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.
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Mutagenesis techniques Boxes 7 176
IDENTIFICATION OF DNABINDING PROTEINS AND ISOLATION OF THEIR GENES
CONCEPTS AND STRATEGIES FOR CLONING GENES ENCODING
Cloning by methods that do not require an initial
CONFIRMING THE FUNCTIONAL IMPORTANCE OF
IN VIVO ANALYSIS OF AN ENDOGENOUS CONTROL REGION
APPROACHES FOR THE SYNTHESIS OF RECOMBINANT TRANSCRIPTION FACTORS
TRANSCRIPTION INITIATION SITE MAPPING
FUNCTIONAL ASSAYS FOR PROMOTER ANALYSIS
Common reporter enzyme assays
IDENTIFICATION AND ANALYSIS OF DISTANT CONTROL REGIONS
Functional assays for the characterization of distant control regions
IDENTIFYING csACTING DNA ELEMENTS WITHIN A CONTROL REGION
IDENTIFYING AND CHARACTERIZING TRANSCRIPTION FACTOR DOMAINS
THEORY CHARACTERIZATION AND MODELING OF DNA BINDING
CRUDE AND FRACTIONATED SYSTEMS FOR IN VITRO TRANSCRIPTION
APPROACHES FOR STUDYING TRANSCRIPTION COMPLEX ASSEMBLY
acid activation domain affinity antibody binding biochemical Biol buffer cDNA cell lines centrifugation Chapter chromatin cleavage cloning coactivators concentration containing control elements core promoter database deletion detected determine digestion distant control regions DNA fragment DNA sequence DNA-binding proteins DNase DNase I footprinting drug-resistance elongation EMSA encoding endogenous enhanceosome enhancer eukaryotic experiments functional assay histone holoenzyme hybridization hypersensitive identified Incubate induced isolated l.CR luciferase mammalian mechanism method molecules mRNA mRNA stability mutagenesis mutant mutant analysis nuclear extract nuclear run-on nuclease nucleosome nucleotides oligonucleotide pellet performed plasmid Prepare primer extension probe protein-DNA interaction Protocol purified radiolabeled reaction recombinant regulatory relevant remodeling reporter gene restriction enzyme RNA polymerase RNA polymerase II RNase protection specific stable transfection assay Step strategy structure studies subunits SWI/SNF template TFIIA TFIIB TFIID tion tran transcription factors transcription initiation transcriptional activation transfection transient transfection tube vector vivo wild-type yeast
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