Transcriptional Regulation in Eukaryotes: Concepts, Strategies, and Techniques

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CSHL Press, Jan 1, 2001 - Science - 640 pages
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In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. Transcriptional Regulation in Eukaryotes answers that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. This important and unique book is essential reading for anyone pursuing the analysis of gene expression in model systems or disease states.
 

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Contents

Mutagenesis techniques Boxes 7 176
238
IDENTIFICATION OF DNABINDING PROTEINS AND ISOLATION OF THEIR GENES
249
CONCEPTS AND STRATEGIES FOR CLONING GENES ENCODING
272
Cloning by methods that do not require an initial
283
CONFIRMING THE FUNCTIONAL IMPORTANCE OF
291
IN VIVO ANALYSIS OF AN ENDOGENOUS CONTROL REGION
319
TECHNIQUES
338
APPROACHES FOR THE SYNTHESIS OF RECOMBINANT TRANSCRIPTION FACTORS
365

TECHNIQUES
87
TRANSCRIPTION INITIATION SITE MAPPING
97
TECHNIQUES
116
FUNCTIONAL ASSAYS FOR PROMOTER ANALYSIS
137
TECHNIQUES
172
Common reporter enzyme assays
180
IDENTIFICATION AND ANALYSIS OF DISTANT CONTROL REGIONS
193
Functional assays for the characterization of distant control regions
205
IDENTIFYING csACTING DNA ELEMENTS WITHIN A CONTROL REGION
213
IDENTIFYING AND CHARACTERIZING TRANSCRIPTION FACTOR DOMAINS
399
PROTEINPROTEIN INTERACTIONS
410
TECHNIQUES
422
THEORY CHARACTERIZATION AND MODELING OF DNA BINDING
433
TECHNIQUES
472
CRUDE AND FRACTIONATED SYSTEMS FOR IN VITRO TRANSCRIPTION
505
APPROACHES FOR STUDYING TRANSCRIPTION COMPLEX ASSEMBLY
579
APPENDICES
617
INDEX
627

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Popular passages

Page 139 - Melton, DA, Krieg, PA, Rebagliati, MR, Maniatis, T., Zinn, K., and Green, MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035-7056.
Page 400 - Smith, DB, and Johnson, KS (1988). Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
Page 195 - Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
Page 138 - Frohman, MA, Dush, MK, and Martin, GR (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998-9002. 2. Edwards, JBDM, Delort, J., and Mallet, J. (1991) Oligodeoxyribonucleotide ligation to single-stranded cDNAs: A new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
Page 293 - Dignam, JD, Lebovitz, RM, and Roeder, RG (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489.
Page 51 - AJ (1992) The mdm-2 oncogene product forms a complex with the p53 protein and inhibits p53-mediated transactivation. Cell 69, 1237-1245.
Page 47 - Brownell, JE, Zhou, J., Ranalli, T, Kobayashi, R., Edmondson, DG, Roth, SY, and Allis, CD (1996). Tetrahymena histone acetyltransferase A: A homolog to yeast Gcn5p linking histone acetylation to gene activation. Cell 84, 843-851.
Page 194 - Neumann E., Schaefer-Ridder M., Wang Y., and Hofschneider P. H.: 1982. Gene transfer into mouse lyoma cells by electroporation in high electric fields.
Page 49 - Roeder RG (1999) Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators. Mol Cell...
Page 47 - Dev. 10:711. . 1997. The downstream core promoter element, DPE, is conserved from Drosophila to humans and is recognized by TAF,,60 of Drosophila.

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