A Study of Abortive Infection in a Mutant Strain of Micrococcus Lysodeikticus Infected with a Bacteriophage |
Contents
MATERIALS | 5 |
METHODS 812 | 8 |
EXPERIMENTAL PROCEDURES AND RESULTS 1249 | 12 |
4 other sections not shown
Common terms and phrases
53-20 was infected abortive infection adsorbed adsorption aeration antiserum approximately Assay Assay Assay bacterium Carbon Dioxide Evolution cells of ML chloroform coli culture ML Cultures of ML digestion diluted distilled water DNA Determinations enzymes extracts five minutes flasks genetic grams increase in DNA incubated Infected Cultures infected with Nl infecting ML infection of ML infective centers infective phage Inoculation intervals latter Lowry Micro lysates lysed lysis lysozyme ML 1 culture ML 53-20 infected ml aliquots mutant N1 and N6 N6 and N1 N6 INFECTED N6 phage Nessler's reagent nitrogen OPTICAL DENSITIES Oxygen Consumption particles per ml phage maturation phage when infected Phages N1 plaques when plated plated with ML prepared produce plaques progeny Protein Kjeldahl PROTEIN SYNTHESIS Reagent recovery of infective RIBONUCLEIC ACID room temperature sample Servall shaker at 32°C simultaneous infection soft agar solution supernatants test tubes titer turbidity Uninfected and Infected UNINFECTED CONTROL vegetative phage