Plasmid Interactions and Gene Amplification in Bacterial Cells |
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Contents
Cointegrate Formation Between Homologous | 14 |
Amplification of NR1 Derivatives | 52 |
Sequences Other Than IS Elements | 92 |
4 other sections not shown
Common terms and phrases
agarose gel Agarose gel electrophoresis ampicillin resistance amplification frequency amplification of NR1 amplified NR1 antibiotic resistance antibiotic resistance genes Apr transconjugants bacterial Bacteriol bands cam DNA cells cultured chromosome clones cointegrate formation concentrations of neomycin containing 200 yg containing amplified plasmids copy number cotransfer frequency cultured in medium deletion mutants DNA sequences DNA was isolated donor EcoRI fragment Escherichia coli factor Figure fragment G gene amplification genetic high concentration homologous recombination host isolated from cells lane lification mating mirabilis mutants neo/kan determinants neo/kan gene neomycin per ml non-amplified novel fragment plasmid DNA plates containing 200 plating efficiency Proteus mirabilis PstI r-determinant of NR1 recombination sites region replication Restriction endonuclease restriction endonuclease digestion RTF-Tc Sal I fragment selection sequence homology spread onto plates stationary-phase strain streptomycin tandem duplication tandem r-determinants thymine Tn_3 Tn3_ trans transposon unamplified undergo amplification wild type NR1 yg of neomycin yg/ml