Molecular Cloning: A Laboratory Manual, Book 3 |
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Results 1-3 of 13
Page 18-54
... bromophenol blue reaches the bottom of the resolving gel ( about 4 hours ) . Then turn off the power supply . 11. Remove the glass plates from the electrophoresis apparatus and place them on a paper towel . Using a spatula , pry the ...
... bromophenol blue reaches the bottom of the resolving gel ( about 4 hours ) . Then turn off the power supply . 11. Remove the glass plates from the electrophoresis apparatus and place them on a paper towel . Using a spatula , pry the ...
Page B-25
... bromophenol blue Formamide : Many batches of reagent - grade formamide are sufficiently pure to be used without further treatment . However , if any yellow color is present , the formamide should be deionized by stirring on a magnetic ...
... bromophenol blue Formamide : Many batches of reagent - grade formamide are sufficiently pure to be used without further treatment . However , if any yellow color is present , the formamide should be deionized by stirring on a magnetic ...
Page F-10
... bromophenol blue , but not xylene cyanol FF [ see Appendix B ] ) , and apply the mixture directly to the column without further treatment . Start collecting fractions ( ~ 125 μl ) immediately after the DNA is applied to the column ...
... bromophenol blue , but not xylene cyanol FF [ see Appendix B ] ) , and apply the mixture directly to the column without further treatment . Start collecting fractions ( ~ 125 μl ) immediately after the DNA is applied to the column ...
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Common terms and phrases
Acad acetate acrylamide activity agarose aliquots amount antibody antigen aqueous phase assay autoclaving autoradiographic bacterial bacteriophage T4 DNA BamHI Biol blunt-ended cDNA cell lines centrifugation chloramphenicol chromatography cloned gene coli DNA polymerase column containing culture Dissolve dithiothreitol DNA ligase DNA polymerase dNTPs double-stranded EDTA EDTA pH 8.0 efficiency electrophoresis eluted encoding Escherichia coli ethanol ethidium bromide eukaryotic expression vectors extract gel-loading buffer H₂O hybridization Incubate intensifying screen ligation linkers mammalian cells medium method mg/ml microfuge tube mixture mRNAs NaCl Natl nitrocellulose nitrocellulose filter nucleic acid nucleotides OH OH P.O. Box pellet phosphate pipette plasmid PMSF polycloning precipitation prepared Proc promoter radioactive radiolabeled reaction remove restriction enzyme room temperature sample sequences sodium sterile stock solution stored strain supernatant target protein Telephone termini tion transcription transfection transfer Tris Cl pH vectors washed western blotting µg/ml