Fundamentals of EnzymologyNow in a new edition, this book provides an up-to-date account of the field of enzymology. it describes the action of enzymes in systems of increasing complexity, and shows how these properties can be best utilized, both clinically and technically. The reader will find in-depth coverage purified enzymes, including full treatment of purification methods, structure, kinetics, mechanism of action, and the control of enzyme activity. The behavior of enzymes in more complex systems is discussed, and the intact cell is thoroughy examined. Fundamentals of Enzymology also provides complete information on enzyme turnover in vivo, clinical aspects of enzymes, and enzyme technology. This new edition pays close attention to new applications of recombinant-DNA technology, and includes the latest information on the production of enzymes, the determination of amin-acid sequences, and the analysis of enzyme structure and function using site-directed mutagenesis. |
Contents
The purification of enzymes | 17 |
References | 52 |
The structure of enzymes | 56 |
Copyright | |
10 other sections not shown
Common terms and phrases
6-phosphate acetyl-CoA adenosinetriphosphatase adenylate amino acids amino-acid side-chains aminotransferase arginine aspartate binding Biochemistry biol Biophys carbamoyl phosphate carbamoyltransferase catalysed catalytic activity cell cent changes Chapter Chem chromatography chymotrypsin coli concentration constant covalent cytosol D-fructose D-glucose degradation dissociation domain enzyme activity enzyme kinetics enzyme-catalysed reactions Enzymes 3rd edn equilibrium example fragments fructose-bisphosphatase fructose-bisphosphate aldolase glucose glutamate glycogen H₂O hexokinase hydrolysis inactivation inhibition inhibitor interactions intracellular involved isoenzyme isolated isomerase kinetics lactate dehydrogenase ligand liver lysosomes malate malate dehydrogenase mechanism membrane metabolic pathways metabolites Methods Enzymol mitochondria molecules multienzyme complex multienzyme protein muscle NAD+ NADH NADP+ nucleotides nucleotidyltransferase occur ornithine orthophosphate oxaloacetate oxidation peptide phosphatase polypeptide polypeptide chain proteases purification pyruvate dehydrogenase reaction catalysed reference Section sequence serum specificity step structure studies substrate subunits synthesis synthetase tissue Trends Biochem trypsin tryptophan vivo Vmax X-ray yeast