Molecular Cloning: A Laboratory Manual, Volume 1

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Cold Spring Harbor Laboratory Press, 2001 - Science - 2344 pages
8 Reviews

The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.
In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques.
The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.
The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions.
The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.

As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

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LibraryThing Review

User Review  - iayork - LibraryThing

the BIBLE of every biologist: So few and so much to say about this bible of Biology at the bench... You'll really find everything you want in it, including the composition of all the buffers and ... Read full review

Review: Molecular Cloning: A Laboratory Manual

User Review  - Adrianna - Goodreads

Important book for someone in molecular biology field Read full review

Other editions - View all

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About the author (2001)

Sambrook was a senior staff scientist at Cold Spring Harbor Laboratory from 1969-1985. He is Professor of Research at the Peter MacCallum Cancer Centre, Melbourne.

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