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0.19 umole pregnenolone 33-hydroxysteroid dehydrogenase activity a,3-unsaturated ketones A5-33-hydroxysteroid dehydrogenase activity acid deficient adrenal acid deficient animals acid deficient diet acid deficient guinea acid transhydrogenase activity activity in guinea acute ascorbic acid addition of ascorbic addition of NADH adrenal ascorbic acid adrenal tissue ascorbic acid concentration ascorbic acid deficient basic system beef adrenal Biochim Biophys bovine chronic ascorbic acid cofactor control adrenal glands control and chronic control animals conversion of pregnenolone corpus luteum deficient adrenal glands deficient adrenal preparations deficient guinea pigs dehydroepiandrosterone depressed synthesis dichloromethane effect of ascorbic enzyme activity estrus cycle experimental animals Figure VII guinea pig adrenal hydroxysteroid dehydrogenase incubation Koritz 28 Kowal mg/100 gm tissue microsomal fraction NADH and ascorbic NADH concentration NADH inhibition NADH-monodehydroascorbic acid transhydrogenase nicotinamide adenine dinucleotide nucleotide oxidation pig adrenal glands pregnenolone to progesterone presence of ascorbic production pyridine steroid dehydrogenase steroidogenesis synthesis of progesterone Tris Buffer ug progesterone/mg protein vitro