Cell Growth and Apoptosis: A Practical ApproachGeorge P. Studzinski This Practical Approach volume is unique in describing the key concepts of cell growth and cell death, particularly by apoptosis, and in providing detailed methods to study these processes. The chapters are written by experts in the field and cover a wide range of important subject areas including cell-cycle traverse, chromosomal abnormalities detected at cell division, DNA damage, and cell death or senescence. The establishment of cell lines and optimization of cell proliferation by growth factors are also described. The result is an invaluable laboratory manual for studies of growth and its abnormalities in mammalian cells. Cell Growth and Apoptosis: A Practical Approach provides state-of-the-art protocols for studies of normal and abnormal cell growth, including cancer and other cell-proliferation disorders, together with a critical appraisal of the various methodologies in use. Its coverage of human cells will make it particularly useful for health professionals. |
Contents
Morphological and biochemical criteria | 7 |
Quiescence of cells | 17 |
Correlated analysis of cyclin B1 and cell cycle | 40 |
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Common terms and phrases
3H]thymidine acid agar aggregates analysis aneuploid antigen apoptosis apoptotic apoptotic cells autoradiography biochemical BrdUrd buffer Cancer cell cycle cell death cell lines cell population cell suspension cellular centrifuge chromatin chromosome colonies crosslinks culture medium curve cytogenetic cytoplasmic cytotoxic Darzynkiewicz debris described in Protocol detection dilution diploid distilled water DNA content DNA filter elution DNA fragmentation DNA synthesis drug drug-treated EDTA elution solution ethanol filter elution assays flasks flow cytometry fluorescence fraction G₁ gene growth factors growth medium haematoxylin histogram immortal Incubate internal standard cells labelled leukaemias leukaemic cells lysis measure membrane method methylcellulose mitosis mitotic necrosis nuclear nuclei number of cells pellet phase plates preparation procedure proliferation protein reagents Resuspend Rinse room temperature S-phase samples SCID mouse Section senescent serum slides staining stock solution strand breaks thymidine tissue culture Traganos trypsin tube tumour cells Wash µg/ml