Cell Growth and Apoptosis: A Practical Approach
George P. Studzinski
IRL Press at Oxford University Press, 1995 - Apoptosis - 269 pages
This Practical Approach volume is unique in describing the key concepts of cell growth and cell death, particularly by apoptosis, and in providing detailed methods to study these processes. The chapters are written by experts in the field and cover a wide range of important subject areas including cell-cycle traverse, chromosomal abnormalities detected at cell division, DNA damage, and cell death or senescence. The establishment of cell lines and optimization of cell proliferation by growth factors are also described. The result is an invaluable laboratory manual for studies of growth and its abnormalities in mammalian cells.
Cell Growth and Apoptosis: A Practical Approach provides state-of-the-art protocols for studies of normal and abnormal cell growth, including cancer and other cell-proliferation disorders, together with a critical appraisal of the various methodologies in use. Its coverage of human cells will make it particularly useful for health professionals.
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Measuring parameters of growth
Quiescence of cells
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acid agar aggregates analysis aneuploid apoptosis apoptotic apoptotic cells autoradiography BrdUrd buffer Cancer cell cycle cell death cell growth cell lines cell population cell suspension cell types cellular centrifuge chromosome colonies coverslips culture medium cytogenetic cytoplasmic cytotoxicity Darzynkiewicz debris described in Protocol dilution diploid dish distilled water DNA content DNA filter elution DNA fragmentation DNA synthesis elution assays ethanol fibroblasts flasks flow cytometry fluorescence fraction gene growth factors growth medium H]thymidine haematoxylin histogram Hoechst human IGF-I immortal increase Incubate labelled laboratory leukaemia leukaemic cells lifespan lysis measure method methylcellulose mitogens mitosis mitotic Molecular normal nuclear nuclei number of cells PDGF pellet phase plates preparation procedure proliferation protein reagents receptor Resuspend Rinse room temperature S-phase samples SCID mouse sections senescent serum slides staining stock solution strand breaks thymidine tissue culture Traganos trypsin tube Wash