Animal Cell Culture: A Practical Approach
R. Ian Freshney
IRL Press at Oxford University Press, 1992 - Cell culture. - 329 pages
Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetricindicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presentingtechniques in a readily accessible form, with detailed protocos given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.
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