Chromatography: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and techniques
Elsevier, Apr 16, 2004 - Science - 584 pages
Chromatography has emerged as the most important and versatile analytical method. The book is not only an updated version of Heftmann's classical text, but it covers areas of future importance, such as microfluidics and computer resources. Under his experienced guidance, authorities in each field have contributed their practical experience to an integrated treatment of modern micro analysis.
In Part A the theoretical basis of individual separation methods is explained and the technical aspects are illustrated. It includes the theory of gas and liquid chromatography as well as specific chromatographic techniques, such as size-exclusion, planar, ion, and affinity chromatography as well as various electrokinetic separation techniques. Microfluidics are covered for the first time and useful sources of analytical instruments are listed and evaluated.
1. Each chapter written by an authority
2. Thorough treatment of the theoretical basis of separation methods
3. Practical guide for performing analyses
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acetonitrile acid adsorption affinity Anal analysis analytes anions applications Biochem buffer capillary column Capillary Electrophoresis cation Chem chemical chiral Chromatogr chromatoplate complex components compounds concentration detection detector devices diameter diffusion coefficient Dionex dipole effect electrokinetic eluent elution flow flow-rate fraction gas chromatography gradient HPLC hydrophobic increase injection injector instrument interactions ion-exchange ionization layer LC–MS ligand linear liquid chromatography mass mass spectrometry methanol method microfabricated microfluidic migration mobile phase mobile-phase mode molecules Nyiredy on-line open-tubular OPLC optimization P.G. Righetti packed column parameters particles peak peptides performance Phenomenex Planar Chromatography plate height polar polymeric polymers pressure proteins pump range resolution response retention factor reversed-phase sample selectivity sensitivity separation Silica Silica Silica Silica solubility solute solvent solvent system sorbents spectrometry stationary phase stationary-phase surface techniques temperature typical velocity volume zone