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5-diphenylpyrazine above-described acetic anhydride acetyl acetyl derivative acidified alcoholic extract alcoholic solution alkaline liquid alkaloid amorphous amyl alcohol aqueous liquid aqueous sodium basic lead acetate benzene boiling brown cent Chemical Research Laboratories chloride chloroform colour colourless needles compound consisted contained cotarnine crystalline substance crystallised from alcohol crystals described diminished pressure dissolved distilled under diminished dried emodin ethereal liquid ethereal solution ethyl acetate evaporated examination extracted with ether fatty acids filtrate following result formula fraction glucoside grams gynocardin heated hesperitin homoeriodictyol hot alcohol hydrochloride hydrogen hydrolysis insoluble Iodine value ipuranol isolated ketone latter lead acetate leaflets light petroleum melting and decomposing melting point mixture Neutralization value obtained phenylpyrazine phytosterol portion potassium hydroxide precipitate pyrazine quercetin readily soluble resin resinous material salt seed separated shaken small amount small quantity sodium carbonate sodium hydroxide solid solvent removed sparingly soluble steam sulphuric acid washed Wellcome Chemical Research whence a]D yielded