Practical Electron Microscopy: A Beginner's Illustrated Guide
Cambridge University Press, Sep 24, 1993 - Science - 173 pages
This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the laboratory technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline "tricks of the trade". The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semithin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immunoelectron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples.
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Dehydration and Embedding
The Electron Microscope
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30 minutes acetone acid alignment anode antibody antigen astigmatism beam BEEM capsule Bendayan block face bottle bottom C2 lens Canada cells Chemicals and Equipment clean colloidal gold condenser lens containing contrast dehydration dental wax developer Dilute dissecting needle distilled water electron microscopy embedding emulsion ethanol exposure Figure filament film filter fixation fixative fixer glass glass knife glutaraldehyde gold particles grid Hayat illumination immunoglobulins incubation infiltration Kodak labeling lead citrate lenses light magnification membranes microscope microtome mol/L cacodylate buffer negative Notes on Method objective aperture objective lens osmium tetroxide P.O. Box paper Pasteur pipettes Petri dish plastic postfixed protein A-gold complex razor blade reagents reduced Remove Rinse room temperature safelight screen semithin sections silver slide sodium hydroxide solution surface syringe tank techniques thin sections tissue sections transmission electron microscopy ultramicrotome uranyl acetate uranyl nitrate vacuum vapor washing wavelength