Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual

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Cold Spring Harbor Laboratory Press, 1998 - Science - 177 pages
The Cold Spring Harbor Yeast Genetics course has evolved continuously from 1970 by combining essential standard methods with the latest technologies. Its course manual has followed suit, providing the community with techniques of proven robustness and detailed, complete protocols. The 1998 edition incorporates elements from the 1994 and 1990 publications but has a wealth of information on additional techniques such as mitotic recombination and random spore analysis, cytoduction, karyogamy, telomeric silencing, transformation techniques, and isolation of genomic DNA. As yeast studies open up the era of post-sequence biology, this manual remains a primary source of technical guidance for newcomers or the established investigator.

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