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adsorbed adsorbed protein layer adsorption albumin amount of heparin amount of protamine anaphylatoxins anticoagulant antithrombin antithrombin III artificial surface biocompatibility biomaterials blood cell counts blood compatibility buffer C3a des Arg cellulose cellulose membrane classical pathway clinical complement activation complement consumption complement system concentration of platelet Cuprophan membrane Cuprophan particles determined dialysis membranes dialyzer different membranes elution erythrocytes extracorporeal factor XII fibrinogen filter unit free protamine function of circulation hemodialysis heparin heparin and protamine heparin beads heparin concentration heparin levels heparin reversal heparin-adsorbed heparinized plasma high loading protamine human plasma immobilized immunoglobulin incubation leukocytes loading protamine membrane neutrophil ng/ml perfused plasma level plasma proteins plasma samples platelet factor protamine coated membrane protamine coated surface protamine concentration protamine fiber particles protamine filters protamine membrane protamine quantitation protamine-coated Cuprophan protein adsorption prothrombin prothrombin fragment F1+2 saline Scanning electron Scanning electron microscopic standard curve tube unit/ml units of heparin whole blood