Immunochemical Techniques, Part I: Hybridoma Technology and Monoclonal Antibodies: Volume 121: Immunochemical Techniques, Part 1
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
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In Vitro Immunization for the Production of Anti Christopher L Reading
SerumFree Medium for Hybridoma and Parental Jan KovAr
An Improved in Vitro Immunization Procedure for Barbara D Boss
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Acad acid added affinity aminopterin animals anti antibody production antigen antigen-specific antigenic sites antisera ascites ascites fluid assay Atassi BALB/c binding bovine Ig bsMAb buffer cell line centrifugation chromosome clones concentration containing culture supernatants described detected DMEM ELISA epitopes filter fraction fused fusion partners growth haptens human hybridomas human monoclonal antibodies hybrid hybridomas hybridoma cells hybridomas immunization immunogen immunoglobulin Immunol incubated injected light chain lymphoblastoid lymphocytes M. Z. Atassi method mice microtiter microtiter plates Milstein molecules mouse murine myeloma cells Natl Nature London nitrocellulose obtained ouabain parental cells pellet peptides pipet plasmacytoma plates polyethylene glycol prepared Proc procedure produced protein radioimmunoassay reagents resuspended room temperature RPMI screening secreted serum-free SFH medium Sigma solid-phase solution specific spleen cells sterile supernatants synthetic peptides Table technique tested tion tissue culture titer tube tumor vitro washed