Serum diagnosis of syphilis and luetin reactionJ.B. Lippincott Company, 1912 - 306 pages |
Other editions - View all
Common terms and phrases
active amboceptor anti antibody anticomplementary antigen antihuman Bacteriolysis became negative Bedeutung Berl blood blood-serum Bruck butyric acid reaction butyric acid test cent cerebrospinal fluid Chancre clinical complement-fixation Complete hæmolysis congenital syphilis containing Corbus corpuscle suspension cutaneous Deut diagnosis of syphilis disease Ehrlich emulsion erythrocytes fixation test fresh serum globulin guinea-pig hæmolytic hæmolytic amboceptor hæmolytic system human serum immune inactivated serum incubation intravenously Jour keratitis klin latent syphilis lesion lipoids liver Lues Luetin negative Luetin positive luetin reaction luetin test LVII method Mün Noguchi obtained pallidum parasyphilitic patient of Drs plement positive reaction precipitate precipitin prepared present protein quantity rabbits Secondary syphilis sera Serodiagnostik der Syphilis serum diagnosis serum reaction specimen substances symptoms Syphilisreaktion syphilitic antibody syphilitic serum Tabes Tertiary syphilis tion tive treatment Treponema pallidum tube Ueber Umstimmung unit of amboceptor Von Dungern Wasser Wassermann reaction Wassermann test Wassermannschen Reaktion Wien Wochenschr
Popular passages
Page 172 - ... multiple miliary vesicles occasionally form. At the same time a beginning central softening of the papule can be seen. Within the next twentyfour hours the papule changes into a vesicle, filled at first with a semiopaque serum that later becomes definitely purulent. Soon after this the pustule ruptures spontaneously or after slight friction or pressure. The margin of the broken pustule remains indurated, while the defect caused by the escape of the pustular content becomes quickly covered by...
Page 169 - The agar columns, which contain innumerable spirocheta1, are then carefully ground in a sterile mortar. The resulting thick paste is gradually diluted by adding, little by little, the fluid culture, which also contains an enormous mass of the pure organisms. "The dilution is continued until the emulsion becomes perfectly liquid. The preparation is next heated to 60° C. for thirty minutes in a water-bath and then 0.5 per cent, tricresol is added.
Page 68 - ... while 30 to 40 per cent. haemolysis is designated as faintly positive. Neither the weakly positive nor the faintly positive reaction should be accepted as a definite diagnosis of syphilis without the presence of strong clinical evidence in favor of such a diagnosis. In case the reaction should be doubtful, the serum should be re-examined after a period of a week has elapsed, and if necessary several examinations in succession should be made.
Page 224 - ... normal sodium hydroxide. It is necessary to take the precaution to employ for this test only cerebrospinal fluid entirely free from blood. The presence of an increased content of protein in the cerebrospinal fluid is indicated by the appearance of a granular or floccular precipitate, which gradually settles to the bottom of the tube, beneath a clear, supernatant fluid. The velocity and intensity of the reaction vary according to the quantity of the protein contained in a given specimen. The greater...
Page 172 - The beginning and course of this reaction resemble the papular form until about the fourth or fifth day, when the inflammatory processes commence to progress. The surface of the indurated round papule becomes mildly edematous, and multiple miliary vesicles occasionally form. At the same time a beginning central softening of the papule can be seen. Within the next twentyfour hours the papule changes into a vesicle, filled at first with a semiopaque serum that later becomes definitely purulent. Soon...
Page 168 - Pure cultures of several strains of the pallidum are allowed to grow for periods of six, twelve, twentyfour, and fifty days at 37° C. under anaerobic conditions. One set is cultivated in ascitic fluid containing a piece of sterile placenta, and the other in ascitic fluid agar also containing placenta. The lower portion of each solid culture in which a dense growth has occurred is cut out and the tissue removed.
Page 59 - For each test two tubes are required, one in the front row and its control in the rear row. There will also be two pairs of tubes to serve as positive and negative controls. Put into each of two small test-tubes front and rear one drop (0.02 cc) of the serum to be tested from a capillary pipette.1 Add to each tube 0.1 cc of 40 per cent.
Page 172 - Torpid Form. — In rare instances, the injection sites fade away to almost invisible points within three or four days, so that they may be passed over as negative reactions. Sometimes these spots suddenly light up again after 10 days or so and progress to small pustular formation.
Page 172 - Pustular Form.— The beginning and course of this reaction resemble the papular form until about the fourth or fifth day, when the inflammatory processes commence to progress. The surface of the indurated round papule becomes mildly edematous, and multiple miliary vesicles occasionally form. At the same time a beginning central softening of the papule obtains.
Page 92 - C. for 20 minutes and use four drops for the test. The difficulty may be obviated in some cases by collecting specimens of serum to be tested just before meal-time because the anticomplementary substance is closely associated with the absorption of the chyle into the circulation soon after the meal. (3) The quality and quantity of the antigen can also be sources of error. If one uses poor antigen, either there will be no positive reaction at all, or weak positive reactions will be entirely overlooked....