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0.5 per cent alcian blue alcian blue solution alcian blue staining aldehyde aldehyde fuchsin aqueous neutral red azure blocking Blue Blue Blue Blue Blue Negative Blue Negative Negative carboxyl groups cartilage celloidin cent acetic acid cent alcian blue cent aqueous neutral chloride Chondroitin sulphate clear and mount connective tissue connective tissue mucins counterstain De-paraffinize sections Dehydrate demonstration diastase diastase digestion distilled water dyes electrolyte enzyme epithelial mucins FIMLT following technique given mucin goblet cells haematoxylin heparan sulphate hexamine hours at 37°C hyaluronic acid hyaluronidase keratan sulphate Magenta mast cell metachromasia metachromatic method methylation minutes mucins stain natural or synthetic Negative Negative Blue Negative Negative Negative neutral mucins neutral red non-sulphated acid mucins Nuclei periodic acid pH 2.5 alcian positive control section purple reaction reactive Schiff's reagent sections and take sialidase sialomucin Spicer stain with alcian strongly sulphated mucins sulphate groups synthetic resin test section type of mucin weakly sulphated