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0N type mlg ability affinity maturation affinity receptors Ag-binding Ag-induced Ag-specific analysis anti-PC anti-u antibody APC function APC performance assays binding activity calcium cell lines cell transfectants stimulated Claflin clonal selection clones co-culture cross-linking data not shown density and affinity detectable double positive down-regulation ELISA Figure FITC flow cytometry Fluo-3AM fluorescence Fluorescence data gene germ line hapten Havran higher affinity hybridoma immune responses Immunol independent of isotype Ionomycin isotype lines with 0N low affinity lower affinity lymphocytes mature B cells mean fluorescence mediate mice migD mIgM mlg expression mlg receptor mlgD mlgD+ mlgM mlgM+D+ mlgM|o mlgMmod molecules mutated in CDR2 PC-BGG PC-BSA PC-OVA PC-PM PC-PM stimulation phosphocholine polysaccharide positive selection pre-stimulation pulsed receptor affinity receptor density receptor internalization receptor number response to PC-PM sample signal transduction single positive somatic hypermutation somatic mutations specific splenic B cells stained stimulated with PC-PM supernatants ug/ml unmutated