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ISOLATION OF CLONES
TUMORPRODUCING CAPACITY OF CELL LINES
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aneuploid antigen approximately arm ratio ascites tumors atypical chromosomes average behavior bi-armed chromosomes Cancer cell line cells per colony centromere chromo chromosome arms chromosome constitution chromosome number chromosomes of Clone clonal lines Clone III Clone IV Clone V tumors colchicine counts culture fluid cytological derived described diameter differences in growth diploid experiment fibroblasts Figure Foley S-180 cells frequency genetic Growth curves growth pattern growth potential growth rate Hauschka host ICR mice incubation indicated inoculation intrinsic growth large numbers lines Clone mean number metacentric metaphase metaphase sets mice morphological mutant near-tetraploid number of cells number of chromosome number of colonies observed obtained octoploid parent cells parent line percentage petri dish Phase contrast Photomicrograph ploidy polyploid progressive growth progressive tumors sarcoma serum single cell small numbers solid tumor stemline studies Table tetraploid tissue culture total number transplantable tumors trypsinization tube tumor growth tumor regression tumor-producing capacities variation vitro vivo