Practice of High Performance Liquid Chromatography: Applications, Equipment and Quantitative AnalysisHeinz Engelhardt During its short 20 year history High Performance Liquid Chro matography (HPLC) has won itself a firm place amongst the instrumental methods of analysis. HPLC has caused a revolution in biological and pharmaceutical chemistry. Approximately two thirds of the publications on HPLC are concerned with problems from this area of life science. Biotechnology, where it is necessary to isolate substances from complicated mixtures, is likely to give further impetus to the dissemination of modern liquid chromatog raphy in columns, particularly on the preparative scale. This book presents, by means of examples, the application of HPLC to various fields, as well as fundamental discussions of chromatographic methods. The quality of the analytical result is decisively dependent on the qualities of the equipment employed (by Colin, Guiochon, and Martin). Especially the demands are discussed that are placed on the components of the instrument including those for data acquisition and processing. The section on "quantitative analy sis" (by ABhauer, Ullner) covers besides the principles also the problems of ensuring the quality of the data in detail. The basic problems arising by enlarging the sample size to preparative di mensions and the requirements put on the aparatus are discussed in the section on "preparative applications" (by Wehrli). |
Contents
| 4 | |
| 6 | |
Sample Introduction Systems | 25 |
Automatization of LC Chromatographs and Instrument Control | 48 |
3 | 60 |
General Points of View and Definitions | 66 |
Quantitative Analysis in HPLC | 67 |
Sources and Representation of Errors | 76 |
Liquidliquid Chromatography | 180 |
Selection of Liquidliquid Systems | 187 |
Applications of LLC | 194 |
Ion Pair Liquid Chromatography | 201 |
Application of HPLC in Inorganic Chemistry | 215 |
HPLC in Forensic Chemistry | 241 |
Application of HPLC to the Separation of Lipids | 286 |
Application of HPLC to the Separation of Metabolites | 323 |
Error Sources in HPLC and their Influence on Quantitation | 85 |
Detectors | 94 |
Practical Experiences | 99 |
References | 108 |
Preparative Application of HPLC | 109 |
H Hulpke U Werthmann | 143 |
Sample Pretreatment and Cleanup | 158 |
Application of HPLC to the Analysis of Natural and Synthetic | 343 |
Application of HPLC for Analysis of Psychotropic Drugs | 392 |
HPLC of Amino Acids and Proteins | 409 |
HPLC in the Separation of Coal and Oil Products | 426 |
| 455 | |
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