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absolute ethanol acetic acid air-dry aliquot allow antibody aqueous autoclaving beaker CaCl2 cells centrifuge tube Chan and Gehring chromosomes comm Dechorionate Dehydrate diluted discs Dissect Dissolve distilled water double-distilled water drop Drosophila Drosophila Ringer's EDTA eggs EGTA embryos ethanol series Euparal Ficoll final concentration fixative flask formaldehyde formamide g/liter gently Giemsa glass glutaraldehyde glycerol hemocytometer homogenizer humid chamber hybridization hypochlorite ig/ml Incubate larvae liquid nitrogen MATERIALS medium G METHOD mg/ml MgCl2 microcentrifuge tube micropipette microscope slides microscope slides coverslips mixture Mount mountant n-heptane N0TES Na0H NaCl needle nuclei overnight paraffin paraformaldehyde Pasteur pipette pellet pers pestle Petri dish phosphate buffer plastic probe Protocol Remove Resuspend Rinse RNase room temperature Schneider's medium siliconized staining sterile stock solution sucrose supernatant Telephone Telex tissue tissue-culture Transfer embryos Tris-HCl Triton twice vial vitelline membrane volume vortex Wash embryos xylene