Bacterial Sensors: Synthetic Design and Application PrinciplesBacterial reporters are live, genetically engineered cells with promising application in bioanalytics. They contain genetic circuitry to produce a cellular sensing element, which detects the target compound and relays the detection to specific synthesis of so-called reporter proteins (the presence or activity of which is easy to quantify). Bioassays with bacterial reporters are a useful complement to chemical analytics because they measure biological responses rather than total chemical concentrations. Simple bacterial reporter assays may also replace more costly chemical methods as a first line sample analysis technique. Recent promising developments integrate bacterial reporter cells with microsystems to produce bacterial biosensors. This lecture presents an in-depth treatment of the synthetic biological design principles of bacterial reporters, the engineering of which started as simple recombinant DNA puzzles, but has now become a more rational approach of choosing and combining sensing, controlling and reporting DNA 'parts'. Several examples of existing bacterial reporter designs and their genetic circuitry will be illustrated. Besides the design principles, the lecture also focuses on the application principles of bacterial reporter assays. A variety of assay formats will be illustrated, and principles of quantification will be dealt with. In addition to this discussion, substantial reference material is supplied in various Annexes. Table of Contents: Short History of the use of Bacteria for Biosensing and Bioreporting / Genetic Engineering Concepts / Measuring with Bioreporters / Epilogue |
Contents
| 1 | |
Genetic Engineering Concepts | 9 |
Measuring with Bioreporters | 71 |
Epilogue | 99 |
Bacterial Bioreporter Designs Targeting Organic Compounds | 101 |
Bacterial Bioreporter Designs Targeting Heavy Metals and Metalloids | 117 |
Bacterial Bioreporter Designs Responsive to Toxicity or Stress Conditions | 131 |
Example Bioreporter Protocols | 141 |
Authors Biography | 153 |
Other editions - View all
Bacterial Sensors: Synthetic Design and Application Principles Jan Roelof van der Meer Limited preview - 2010 |
Bacterial Sensors: Synthetic Design and Application Principles Jan Roelof van der Meer Limited preview - 2022 |
Bacterial Sensors: Synthetic Design and Application Principles Jan Roelof van der Meer No preview available - 2010 |
Common terms and phrases
Appl Application Reference Switch aptamer Aqueous solutions arsenic arsenite ArsR Assay Measurement Detected assay time Luminometry bacterial reporter Bacteriol beta-galactosidase binding bioavailable bioluminescent bioreporter bioreporter assay biosensor Biotechnol chemical chromosome compounds Concentration range Concentration range Application construction Assay Measurement degradation design Host strain Detected compounds Concentration effector Environ epifluorescence microscopy Escherichia coli flippase flow cytometry fusion gene expression gene Reporter design genes Source organism genetic HbpR Homologous host encoded inducer IPTG Leveau luciferase luxAB Measurement Detected compounds MerR metabolism Microbiol molecules mutants naphthalene operon organism Reporter gene phenanthrene plasmid Prom promoter Pseudomonas putida range Application Reference Reference Switch Source regulatory protein reporter cells reporter circuit Reporter design Host Reporter gene Reporter reporter protein reporter signal repressor resistance response RNA polymerase sample Source genes Source Source organism Reporter specific strain for construction Switch Source genes target compound Tecon toluene toxicity transcription activators transposon whole-cell XylR