Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page 1-66
... cleaved with two different restriction enzymes ; stuffer fragment not removed . ( ) Vector cleaved with one restriction enzyme and dephosphorylated . ( △ ) Vector cleaved with one restriction enzyme and not dephosphorylated . This ...
... cleaved with two different restriction enzymes ; stuffer fragment not removed . ( ) Vector cleaved with one restriction enzyme and dephosphorylated . ( △ ) Vector cleaved with one restriction enzyme and not dephosphorylated . This ...
Page 5-3
... cleave double - stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence . These enzymes have been classified into three groups . Type I and type III enzymes ( see Table 5.1 ) carry ...
... cleave double - stranded DNA at specific sites within or adjacent to a particular sequence known as the recognition sequence . These enzymes have been classified into three groups . Type I and type III enzymes ( see Table 5.1 ) carry ...
Page 5-14
... cleaved with BamHI . ( Note : Only 1 in every 16 Mbol / BamHI fusions regenerate a BamHI site , and therefore it is not usually possible to recover an MboI fragment inserted into a BamHI vector by digesting the recombinant with BamHI ...
... cleaved with BamHI . ( Note : Only 1 in every 16 Mbol / BamHI fusions regenerate a BamHI site , and therefore it is not usually possible to recover an MboI fragment inserted into a BamHI vector by digesting the recombinant with BamHI ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
Copyright | |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml