Molecular Cloning: A Laboratory Manual, Volume 1 |
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Page vii
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
... cloning and in the range and power of the techniques used to handle recombinant DNA . This remarkable proliferation of cloning methods is reflected in the number of gene sequences in the GenBank DNA sequence data base . In 1982 there ...
Page xxii
... CLONING OF DOUBLE - STRANDED cDNA 8.21 Homopolymeric Tailing 8.21 Synthetic DNA Linkers and Adapters 8.23 Alternative Methods of Cloning cDNA 8.27 mRNA - cDNA CLONING 8.27 SEQUENTIAL ADDITION OF DIFFERENT LINKERS 8.27 CDNA CLONING IN ...
... CLONING OF DOUBLE - STRANDED cDNA 8.21 Homopolymeric Tailing 8.21 Synthetic DNA Linkers and Adapters 8.23 Alternative Methods of Cloning cDNA 8.27 mRNA - cDNA CLONING 8.27 SEQUENTIAL ADDITION OF DIFFERENT LINKERS 8.27 CDNA CLONING IN ...
Page 1-6
... cloning vectors , 1.60-1.61 treatment of cosmid vector DNA digestion with one restriction enzyme and ... Cloning of DNA in bacteriophage \ , 2.82–2.107 . See also cDNA synthesis and cloning ; \ bacteriophage in cosmid vectors , 3.4-3.6 ...
... cloning vectors , 1.60-1.61 treatment of cosmid vector DNA digestion with one restriction enzyme and ... Cloning of DNA in bacteriophage \ , 2.82–2.107 . See also cDNA synthesis and cloning ; \ bacteriophage in cosmid vectors , 3.4-3.6 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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Common terms and phrases
agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml