Molecular Cloning: A Laboratory Manual, Volume 1 |
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Results 1-3 of 32
Page 1-3
... origin of DNA replication . Usually , a plasmid will contain only one origin of replication together with its associated cis - acting control elements ( the whole genetic unit being defined as a " replicon " ) . Very rarely , however ...
... origin of DNA replication . Usually , a plasmid will contain only one origin of replication together with its associated cis - acting control elements ( the whole genetic unit being defined as a " replicon " ) . Very rarely , however ...
Page 1-9
... Origins of Replication Derived from Single - stranded Bacteriophages Several plasmid vectors have been constructed that carry the origin of replication from the genome of a single - stranded filamentous bacteriophage such as M13 ( see ...
... Origins of Replication Derived from Single - stranded Bacteriophages Several plasmid vectors have been constructed that carry the origin of replication from the genome of a single - stranded filamentous bacteriophage such as M13 ( see ...
Page 3-19
... origin of replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency with which cosmid DNA can be packaged in vivo into transducing particles . The only useful cloning site in pHC79 ...
... origin of replication and two copies of cos oriented in tandem . This arrangement of cos sequences increases the efficiency with which cosmid DNA can be packaged in vivo into transducing particles . The only useful cloning site in pHC79 ...
Contents
DEVELOPMENT OF PLASMID CLONING VECTORS | 1-7 |
Constructing Expression Libraries in Plasmid and Bacteriophage | 1-12 |
Expression Libraries Constructed in Bacteriophage 12 | 1-19 |
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agar plate agarose gel aliquots amber mutations ampicillin antibiotic bacteriophage particles bacteriophage T4 bacteriophage T4 DNA BamHI Bgill buffer carrying cDNA cells cloning coli cosmid cosmid vector culture digestion DNA fragments DNA ligase DNA molecules DNA polymerase double-stranded DNA EcoRI EDTA EDTA pH 8.0 efficiency Escherichia coli ethanol ethidium bromide eukaryotic eukaryotic DNA exonuclease extracts foreign DNA formamide gene genetic HindIII Hindill host hours at 37°C hybridization Incubate infected inserted Kpnl lacZ ligation reaction linear lysogenic method microfuge tube minutes at 4°C nin5 nitrocellulose nitrocellulose filter Nucleic Acids nucleotides packaging pasteur pipette pellet plaques plasmid DNA plasmid vectors polycloning prepared probes protein purified Pvul recA red gam remove replication restriction enzyme RNAase room temperature Sacl Sall sequences Smal solution sterile stored strains strand stuffer supernatant T4 DNA ligase teriophage transfer transformation Tris Cl pH vector DNA vitro volume Xbal µg/ml