What people are saying - Write a review
We haven't found any reviews in the usual places.
TEST LIGATION 3 29
SYNTHESIS OF THE FIRST STRAND OF cDNA 8
88 other sections not shown
Other editions - View all
agarose gel aliquots amino acid amplification antibody appropriate approximately bacterial bacteriophage M13 bacteriophage T4 bacteriophage T4 DNA buffer carried cDNA clones cDNA libraries cells Chapter chromatography Cl pH cleavage cleaves cleotide codon coli DNA polymerase complementary concentration containing deletions denatured described digestion dithiothreitol DNA fragments DNA ligase DNA polymerase DNA sequencing DNAase dNTPs double-stranded DNA EDTA EDTA pH 8.0 efficiency ethanol ethidium bromide exonuclease fusion proteins gel electrophoresis gene genomic hybridization Incubate the reaction inserted Klenow fragment ligation linkers mammalian method MgCl2 microfuge tube minutes at 4°C molecules mRNA mutagenesis mutagenic oligonucleotide mutations Natl nitrocellulose nitrocellulose filter nucleic acids nucleotides oligonucleotide phagemid pipette plaques plasmid plasmid DNA plates prepared primer probe purified radioactivity radiolabeled recombinant remove restriction enzyme room temperature samples screening single-stranded DNA solution specific activity step stored strand of cDNA synthesis T4 DNA ligase target DNA target sequence termini transcription transfer volume Weiss unit