Yeast: A Practical Approach
I. Campbell, John H. Duffus
IRL Press, 1988 - Levure - Manuels de laboratoire - 289 pages
The importance of yeasts in the biotechnology industry has progressed well beyond baking and brewing. Yeasts are now the organisms of choice for the incorporation of mammalian genes for expressing proteins and other products which were previously obtained from animals. Some yeasts, due to their potential to cause intractable diseases, also have medical significance. Yeast provides clear descriptions of the methods used in current research. Written by scientists of great experience, this book provides an important compendium of methods for established researchers. It will also benefit workers new to the field.
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ISOLATION OF YEAST NUCLEI AND CHROMATIN
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acetate agar aliquots allelic antibiotics asci ascus assay autoclaving auxotrophic buffer cell wall cells/ml centrifugation cerevisiae chromosome clones colonies containing coverslip cross cytoplasm digestion dilute diploid dissection dissolve EDTA electron enzyme ethanol ethidium bromide extract Figure filter final concentration fluorescence fractions gene genetic genome glucose gradient grow growth haploid hybridization Incubate at 28°C isolation lipid lysate lysis markers mating membrane method mg/ml microscope mitochondrial molecules mRNA mtDNA mutagenized mutants N3 medium needle nuclear nuclease nuclei Pasteur pipette pellet phase phenotype pipette plasmid preparation procedure protein protoplasts purity recombinant remove replica plated resuspend rho mutants room temperature rotor Sacch sample Section slide sodium sorbitol specimen spheroplasts spores sporulation step sterile water stock solution sucrose supernatant suspension Table techniques tester strains tion transcription transfer Tris-HCl tube ug/ml volume wash yeast yeast cells yeast strains YEP-glucose YPglu Zymolyase