Basic and Clinical Applications of Flow Cytometry: Proceeding of the 24th Annual Detroit Cancer Symposium Detroit, Michigan, USA - April 30, May 1 and 2, 1992
Frederick A. Valeriote, Alexander Nakeff, Manuel Valdivieso
Springer US, Dec 31, 1995 - Medical - 318 pages
The focus of this symposium was on the present and future capabilities of flow cytometry for both medical and biological applications in cancer. This technology began with quite modest instrumentation, with limited capabilities to answer biological questions. Today, both the clinical workhorses and the powerful multi-laser, multi-detector, sorting machinery, coupled with sophisticated computers and storage devices and the increasing storehouse of markers and dyes, are taking us to the limit and beyond in finding answers to the cause and cure of cancer.
In the past, both normal hematopoietic tissue and leukemias have been the tissue samples of choice in the application of flow cytometry, and some of the most recent applications with these tissues are presented here. However, the book also discusses the increasingly sophisticated disaggregation techniques which allow investigators the possibility to train their lasers on solid tumors. Not only can we use flow cytometry with associated fluorescent markers to understand the biology of cancer, but also the wide array of existing and developing markers provides us with important diagnostic tools in the detection of cancer early in either the malignant or relapse process. And the field comes full circle, with the use of the technology for gene mapping and other genetic studies to unlock the basic malignant process.
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DNA TOPOISOMERASE II POISONS AND THE CELL CYCLE
IN SITU HYBRIDIZATION OF CENTROMERIC DNA
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activation antigen Biol bone marrow breast cancer c-myc Cancer Res carcinoma CD4+ T-lymphocytes CD8+ cell cycle cell differentiation cell lines cell proliferation cell sorting cellular chemotherapy chromosome clinical correlated cultures cyclins cytome cytoplasmic cytotoxic debris detection diploid dissociation DNA analysis DNA aneuploid DNA content DNA diploid DNA flow cytometry DNA histograms DNA ploidy doxorubicin drug efflux enzymatic enzyme expression factors fetal cells flow cytometric analysis flow cytometry flow karyotype fluorescence fraction gene head and neck high-speed histograms human Immunol induced intracellular leukemia lymph lymphocytes lymphoma markers megakaryocytes method molecular monoclonal antibodies motility multiparameter murine normal nuclear matrix nuclei oncogene Oncology paraffin-embedded parameters patients phase phenotype ploidy principal component prognostic proliferative protein RCS/Cy mice RCS5 mice receptor response S-phase S-phase fraction samples SCCHN solid tumors sorted cells specific staining stem cells stimulation studies T-lymphocytes techniques therapy tion tissue topoisomerase topoisomerase II tumor tumor cells vitro