Mass Spectrometry of Protein Interactions
John Wiley & Sons, Aug 24, 2007 - Science - 220 pages
The authoritative guide to analyzing protein interactions by mass spectrometry
Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text:
Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS)
Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS)
Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions
Reviews the latest discoveries and applications and addresses new and ongoing challenges
This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.
What people are saying - Write a review
We haven't found any reviews in the usual places.
2 Softly SoftlyDetection of Protein Complexes by MatrixAssisted Laser Desorption Ionization Mass Spectrometry
3 Probing Protein Interactions Using HydrogenDeuterium Exchange Mass Spectrometry
4 Limited Proteolysis Mass Spectrometry of Protein Complexes
Other editions - View all
acid side chains amide hydrogens amino acid amino acid side amyloid fibrils Anal analyzed apomyoglobin approach binding Biochem Biochemistry Biol Bottom–Up calmodulin Chem chemical cross-linking cleavage conformational changes cross-linked products cross-linking cross-linking reagents detection deuterium deuterium uptake DHFR digestion dimer dissociation domain Downard electrospray ionization mass ESI-MS Figure formation gas phase Hillenkamp HX-MS hydrogen exchange hydroxyl radicals identification infrared multiphoton dissociation interface ion mobility ionization mass spectrometry ligand MALDI MALDI-MS Maleknia Mass Spectrom mass spectrometric analysis matrix matrix-assisted laser desorption/ionization measured molecular molecules N-terminal NHS esters noncovalent noncovalent complexes noncovalent protein oxidation oxidative damage peptide protease proteasome protein complexes protein interactions Protein Sci protein structure protein–ligand protein–protein interactions proteolysis proteolytic Proteomics reactive residues regions residue side chains ribonuclease sample SH3 domain side chains solution solvent accessibility spectra study of protein studying protein subunit surface synchrotron target tein tion top–down unfolding X-ray